trna scaffolded sa trsa sequence Search Results


pcdna3 trsa empty vector  (Addgene inc)
93
Addgene inc pcdna3 trsa empty vector
Pcdna3 Trsa Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3 trsa empty vector/product/Addgene inc
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pcdna3 trsa empty vector - by Bioz Stars, 2026-02
93/100 stars
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sialyl-lactose-bound trsa  (Databank Inc)
90
Databank Inc sialyl-lactose-bound trsa
Sialyl Lactose Bound Trsa, supplied by Databank Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sialyl-lactose-bound trsa/product/Databank Inc
Average 90 stars, based on 1 article reviews
sialyl-lactose-bound trsa - by Bioz Stars, 2026-02
90/100 stars
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pcdna3.0-(-1 prf)-trsa  (Promega)
90
Promega pcdna3.0-(-1 prf)-trsa
SLBP bound to the stem loop region of SARS-CoV-2 -1 PRF RNA. a -1 PRF RNA sequences were conserved among the genome sequences of 19 different SARS-CoV-2 strains. The viral sequences were manually retrieved from the National Center for Biotechnology Information (NCBI). Sequence alignment was conducted using MEGA 11.0 software. b The proposed secondary structure of the SARS-CoV-2 -1 PRF element. c Schematic representations of the -1 PRF RNA studied, including full -1 PRF RNA, ΔSL2 mutant, ΔSL3 mutant, ΔSL1& 2 mutant, and ΔSL2&3 mutant. The schematic diagram was generated with IBS 2.0 ( https://ibs.renlab.org/#/server ). d RNA probes used were obtained from in vitro transcription. e Using cellular proteins extracted from H1299 cells, immunoprecipitation of <t>tRSA-labeled</t> wild-type and mutant -1 PRF RNA probes pulled down SLBP. tRSA-labeled random sequence RNA was used as a negative control ( n = 3). f Interaction between SLBP and wild-type -1 PRF RNA or predicted RNA sequence of -1 PRF RNA using RNA EMSAs. Lane 1, -1 PRF RNA truncated mutant I. Lane 2, -1 PRF RNA truncated mutant II. Lane 3, -1 PRF RNA truncated mutant III. Lane 4, -1 PRF RNA. Lane 5, -1 PRF RNA truncated mutant I + GST. Lane 6, -1 PRF RNA truncated mutant II + GST. Lane 7, -1 PRF RNA truncated mutant III + GST. Lane 8, -1 PRF RNA + GST. Lane 9, -1 PRF RNA truncated mutant I + GST-SLBP. Lane 10, -1 PRF RNA truncated mutant II + GST-SLBP. Lane 11, -1 PRF RNA truncated mutant III + GST-SLBP. Lane 12, -1 PRF RNA + GST-SLBP. Experiments were repeated three times with similar results. g Using cellular proteins extracted from H1299 cells, immunoprecipitation of random sequence RNA, wild-type and silent SL3 mutation -1 PRF RNA probes pulled down SLBP. h Schematic diagram of interaction models between SLBP and -1 PRF RNA through the molecular docking. The RNA pull-down followed by WB assay was performed to evaluate the interaction between SLBP mutants and -1 PRF RNA. Total cell lysates from H1299 cells transfected with different SLBP variants were used in the experiment. These variants included single mutations where arginine 46 was changed to alanine (R46A), serine 94 was changed to alanine (S94A), and a double mutation (R46A & S94A), where both arginine 46 and serine 94 were replaced with alanine, respectively. Meanwhile, total cell lysates from H1299 cells transfected with wild-type SLBP (WT) were used as a positive control. i Co-detection of SLBP with -1 PRF RNA. Polyclonal anti-SLBP was used for SLBP protein immunofluorescence. Scale bar = 20 µm. j Schematic diagram of five host factors promoting SARS-CoV-2 replication via regulating frameshifting. SARS-CoV-2 apply the -1 PRF mechanism to switch complete protein translation from ORF1a to ORF1b, while FUBP3, SLBP, RPL10A, RPS3A, and RPS14 bind directly to the SARS-CoV-2 -1 PRF RNA to promote replication via enhancing the switch procedure. The expression of SFL inhibited SARS-CoV-2 replication via reducing frameshifting. The schematic diagram was generated with www.figdraw.com . Data points represent the mean ± SEM. Multiple comparisons were performed using ANOVA with Dunnett’s test. * P < 0.05, ** P < 0.01, and *** P < 0.001
Pcdna3.0 ( 1 Prf) Trsa, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3.0-(-1 prf)-trsa/product/Promega
Average 90 stars, based on 1 article reviews
pcdna3.0-(-1 prf)-trsa - by Bioz Stars, 2026-02
90/100 stars
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recombinant trsa  (Thermo Fisher)
90
Thermo Fisher recombinant trsa
SLBP bound to the stem loop region of SARS-CoV-2 -1 PRF RNA. a -1 PRF RNA sequences were conserved among the genome sequences of 19 different SARS-CoV-2 strains. The viral sequences were manually retrieved from the National Center for Biotechnology Information (NCBI). Sequence alignment was conducted using MEGA 11.0 software. b The proposed secondary structure of the SARS-CoV-2 -1 PRF element. c Schematic representations of the -1 PRF RNA studied, including full -1 PRF RNA, ΔSL2 mutant, ΔSL3 mutant, ΔSL1& 2 mutant, and ΔSL2&3 mutant. The schematic diagram was generated with IBS 2.0 ( https://ibs.renlab.org/#/server ). d RNA probes used were obtained from in vitro transcription. e Using cellular proteins extracted from H1299 cells, immunoprecipitation of <t>tRSA-labeled</t> wild-type and mutant -1 PRF RNA probes pulled down SLBP. tRSA-labeled random sequence RNA was used as a negative control ( n = 3). f Interaction between SLBP and wild-type -1 PRF RNA or predicted RNA sequence of -1 PRF RNA using RNA EMSAs. Lane 1, -1 PRF RNA truncated mutant I. Lane 2, -1 PRF RNA truncated mutant II. Lane 3, -1 PRF RNA truncated mutant III. Lane 4, -1 PRF RNA. Lane 5, -1 PRF RNA truncated mutant I + GST. Lane 6, -1 PRF RNA truncated mutant II + GST. Lane 7, -1 PRF RNA truncated mutant III + GST. Lane 8, -1 PRF RNA + GST. Lane 9, -1 PRF RNA truncated mutant I + GST-SLBP. Lane 10, -1 PRF RNA truncated mutant II + GST-SLBP. Lane 11, -1 PRF RNA truncated mutant III + GST-SLBP. Lane 12, -1 PRF RNA + GST-SLBP. Experiments were repeated three times with similar results. g Using cellular proteins extracted from H1299 cells, immunoprecipitation of random sequence RNA, wild-type and silent SL3 mutation -1 PRF RNA probes pulled down SLBP. h Schematic diagram of interaction models between SLBP and -1 PRF RNA through the molecular docking. The RNA pull-down followed by WB assay was performed to evaluate the interaction between SLBP mutants and -1 PRF RNA. Total cell lysates from H1299 cells transfected with different SLBP variants were used in the experiment. These variants included single mutations where arginine 46 was changed to alanine (R46A), serine 94 was changed to alanine (S94A), and a double mutation (R46A & S94A), where both arginine 46 and serine 94 were replaced with alanine, respectively. Meanwhile, total cell lysates from H1299 cells transfected with wild-type SLBP (WT) were used as a positive control. i Co-detection of SLBP with -1 PRF RNA. Polyclonal anti-SLBP was used for SLBP protein immunofluorescence. Scale bar = 20 µm. j Schematic diagram of five host factors promoting SARS-CoV-2 replication via regulating frameshifting. SARS-CoV-2 apply the -1 PRF mechanism to switch complete protein translation from ORF1a to ORF1b, while FUBP3, SLBP, RPL10A, RPS3A, and RPS14 bind directly to the SARS-CoV-2 -1 PRF RNA to promote replication via enhancing the switch procedure. The expression of SFL inhibited SARS-CoV-2 replication via reducing frameshifting. The schematic diagram was generated with www.figdraw.com . Data points represent the mean ± SEM. Multiple comparisons were performed using ANOVA with Dunnett’s test. * P < 0.05, ** P < 0.01, and *** P < 0.001
Recombinant Trsa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant trsa/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
recombinant trsa - by Bioz Stars, 2026-02
90/100 stars
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step scan trss ftir ds  (Bruker Corporation)
95
Bruker Corporation step scan trss ftir ds
SLBP bound to the stem loop region of SARS-CoV-2 -1 PRF RNA. a -1 PRF RNA sequences were conserved among the genome sequences of 19 different SARS-CoV-2 strains. The viral sequences were manually retrieved from the National Center for Biotechnology Information (NCBI). Sequence alignment was conducted using MEGA 11.0 software. b The proposed secondary structure of the SARS-CoV-2 -1 PRF element. c Schematic representations of the -1 PRF RNA studied, including full -1 PRF RNA, ΔSL2 mutant, ΔSL3 mutant, ΔSL1& 2 mutant, and ΔSL2&3 mutant. The schematic diagram was generated with IBS 2.0 ( https://ibs.renlab.org/#/server ). d RNA probes used were obtained from in vitro transcription. e Using cellular proteins extracted from H1299 cells, immunoprecipitation of <t>tRSA-labeled</t> wild-type and mutant -1 PRF RNA probes pulled down SLBP. tRSA-labeled random sequence RNA was used as a negative control ( n = 3). f Interaction between SLBP and wild-type -1 PRF RNA or predicted RNA sequence of -1 PRF RNA using RNA EMSAs. Lane 1, -1 PRF RNA truncated mutant I. Lane 2, -1 PRF RNA truncated mutant II. Lane 3, -1 PRF RNA truncated mutant III. Lane 4, -1 PRF RNA. Lane 5, -1 PRF RNA truncated mutant I + GST. Lane 6, -1 PRF RNA truncated mutant II + GST. Lane 7, -1 PRF RNA truncated mutant III + GST. Lane 8, -1 PRF RNA + GST. Lane 9, -1 PRF RNA truncated mutant I + GST-SLBP. Lane 10, -1 PRF RNA truncated mutant II + GST-SLBP. Lane 11, -1 PRF RNA truncated mutant III + GST-SLBP. Lane 12, -1 PRF RNA + GST-SLBP. Experiments were repeated three times with similar results. g Using cellular proteins extracted from H1299 cells, immunoprecipitation of random sequence RNA, wild-type and silent SL3 mutation -1 PRF RNA probes pulled down SLBP. h Schematic diagram of interaction models between SLBP and -1 PRF RNA through the molecular docking. The RNA pull-down followed by WB assay was performed to evaluate the interaction between SLBP mutants and -1 PRF RNA. Total cell lysates from H1299 cells transfected with different SLBP variants were used in the experiment. These variants included single mutations where arginine 46 was changed to alanine (R46A), serine 94 was changed to alanine (S94A), and a double mutation (R46A & S94A), where both arginine 46 and serine 94 were replaced with alanine, respectively. Meanwhile, total cell lysates from H1299 cells transfected with wild-type SLBP (WT) were used as a positive control. i Co-detection of SLBP with -1 PRF RNA. Polyclonal anti-SLBP was used for SLBP protein immunofluorescence. Scale bar = 20 µm. j Schematic diagram of five host factors promoting SARS-CoV-2 replication via regulating frameshifting. SARS-CoV-2 apply the -1 PRF mechanism to switch complete protein translation from ORF1a to ORF1b, while FUBP3, SLBP, RPL10A, RPS3A, and RPS14 bind directly to the SARS-CoV-2 -1 PRF RNA to promote replication via enhancing the switch procedure. The expression of SFL inhibited SARS-CoV-2 replication via reducing frameshifting. The schematic diagram was generated with www.figdraw.com . Data points represent the mean ± SEM. Multiple comparisons were performed using ANOVA with Dunnett’s test. * P < 0.05, ** P < 0.01, and *** P < 0.001
Step Scan Trss Ftir Ds, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/step scan trss ftir ds/product/Bruker Corporation
Average 95 stars, based on 1 article reviews
step scan trss ftir ds - by Bioz Stars, 2026-02
95/100 stars
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pcdna3 trsa  (Addgene inc)
91
Addgene inc pcdna3 trsa
SLBP bound to the stem loop region of SARS-CoV-2 -1 PRF RNA. a -1 PRF RNA sequences were conserved among the genome sequences of 19 different SARS-CoV-2 strains. The viral sequences were manually retrieved from the National Center for Biotechnology Information (NCBI). Sequence alignment was conducted using MEGA 11.0 software. b The proposed secondary structure of the SARS-CoV-2 -1 PRF element. c Schematic representations of the -1 PRF RNA studied, including full -1 PRF RNA, ΔSL2 mutant, ΔSL3 mutant, ΔSL1& 2 mutant, and ΔSL2&3 mutant. The schematic diagram was generated with IBS 2.0 ( https://ibs.renlab.org/#/server ). d RNA probes used were obtained from in vitro transcription. e Using cellular proteins extracted from H1299 cells, immunoprecipitation of <t>tRSA-labeled</t> wild-type and mutant -1 PRF RNA probes pulled down SLBP. tRSA-labeled random sequence RNA was used as a negative control ( n = 3). f Interaction between SLBP and wild-type -1 PRF RNA or predicted RNA sequence of -1 PRF RNA using RNA EMSAs. Lane 1, -1 PRF RNA truncated mutant I. Lane 2, -1 PRF RNA truncated mutant II. Lane 3, -1 PRF RNA truncated mutant III. Lane 4, -1 PRF RNA. Lane 5, -1 PRF RNA truncated mutant I + GST. Lane 6, -1 PRF RNA truncated mutant II + GST. Lane 7, -1 PRF RNA truncated mutant III + GST. Lane 8, -1 PRF RNA + GST. Lane 9, -1 PRF RNA truncated mutant I + GST-SLBP. Lane 10, -1 PRF RNA truncated mutant II + GST-SLBP. Lane 11, -1 PRF RNA truncated mutant III + GST-SLBP. Lane 12, -1 PRF RNA + GST-SLBP. Experiments were repeated three times with similar results. g Using cellular proteins extracted from H1299 cells, immunoprecipitation of random sequence RNA, wild-type and silent SL3 mutation -1 PRF RNA probes pulled down SLBP. h Schematic diagram of interaction models between SLBP and -1 PRF RNA through the molecular docking. The RNA pull-down followed by WB assay was performed to evaluate the interaction between SLBP mutants and -1 PRF RNA. Total cell lysates from H1299 cells transfected with different SLBP variants were used in the experiment. These variants included single mutations where arginine 46 was changed to alanine (R46A), serine 94 was changed to alanine (S94A), and a double mutation (R46A & S94A), where both arginine 46 and serine 94 were replaced with alanine, respectively. Meanwhile, total cell lysates from H1299 cells transfected with wild-type SLBP (WT) were used as a positive control. i Co-detection of SLBP with -1 PRF RNA. Polyclonal anti-SLBP was used for SLBP protein immunofluorescence. Scale bar = 20 µm. j Schematic diagram of five host factors promoting SARS-CoV-2 replication via regulating frameshifting. SARS-CoV-2 apply the -1 PRF mechanism to switch complete protein translation from ORF1a to ORF1b, while FUBP3, SLBP, RPL10A, RPS3A, and RPS14 bind directly to the SARS-CoV-2 -1 PRF RNA to promote replication via enhancing the switch procedure. The expression of SFL inhibited SARS-CoV-2 replication via reducing frameshifting. The schematic diagram was generated with www.figdraw.com . Data points represent the mean ± SEM. Multiple comparisons were performed using ANOVA with Dunnett’s test. * P < 0.05, ** P < 0.01, and *** P < 0.001
Pcdna3 Trsa, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3 trsa/product/Addgene inc
Average 91 stars, based on 1 article reviews
pcdna3 trsa - by Bioz Stars, 2026-02
91/100 stars
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oligos trsa  (Oligos Etc)
90
Oligos Etc oligos trsa
SLBP bound to the stem loop region of SARS-CoV-2 -1 PRF RNA. a -1 PRF RNA sequences were conserved among the genome sequences of 19 different SARS-CoV-2 strains. The viral sequences were manually retrieved from the National Center for Biotechnology Information (NCBI). Sequence alignment was conducted using MEGA 11.0 software. b The proposed secondary structure of the SARS-CoV-2 -1 PRF element. c Schematic representations of the -1 PRF RNA studied, including full -1 PRF RNA, ΔSL2 mutant, ΔSL3 mutant, ΔSL1& 2 mutant, and ΔSL2&3 mutant. The schematic diagram was generated with IBS 2.0 ( https://ibs.renlab.org/#/server ). d RNA probes used were obtained from in vitro transcription. e Using cellular proteins extracted from H1299 cells, immunoprecipitation of <t>tRSA-labeled</t> wild-type and mutant -1 PRF RNA probes pulled down SLBP. tRSA-labeled random sequence RNA was used as a negative control ( n = 3). f Interaction between SLBP and wild-type -1 PRF RNA or predicted RNA sequence of -1 PRF RNA using RNA EMSAs. Lane 1, -1 PRF RNA truncated mutant I. Lane 2, -1 PRF RNA truncated mutant II. Lane 3, -1 PRF RNA truncated mutant III. Lane 4, -1 PRF RNA. Lane 5, -1 PRF RNA truncated mutant I + GST. Lane 6, -1 PRF RNA truncated mutant II + GST. Lane 7, -1 PRF RNA truncated mutant III + GST. Lane 8, -1 PRF RNA + GST. Lane 9, -1 PRF RNA truncated mutant I + GST-SLBP. Lane 10, -1 PRF RNA truncated mutant II + GST-SLBP. Lane 11, -1 PRF RNA truncated mutant III + GST-SLBP. Lane 12, -1 PRF RNA + GST-SLBP. Experiments were repeated three times with similar results. g Using cellular proteins extracted from H1299 cells, immunoprecipitation of random sequence RNA, wild-type and silent SL3 mutation -1 PRF RNA probes pulled down SLBP. h Schematic diagram of interaction models between SLBP and -1 PRF RNA through the molecular docking. The RNA pull-down followed by WB assay was performed to evaluate the interaction between SLBP mutants and -1 PRF RNA. Total cell lysates from H1299 cells transfected with different SLBP variants were used in the experiment. These variants included single mutations where arginine 46 was changed to alanine (R46A), serine 94 was changed to alanine (S94A), and a double mutation (R46A & S94A), where both arginine 46 and serine 94 were replaced with alanine, respectively. Meanwhile, total cell lysates from H1299 cells transfected with wild-type SLBP (WT) were used as a positive control. i Co-detection of SLBP with -1 PRF RNA. Polyclonal anti-SLBP was used for SLBP protein immunofluorescence. Scale bar = 20 µm. j Schematic diagram of five host factors promoting SARS-CoV-2 replication via regulating frameshifting. SARS-CoV-2 apply the -1 PRF mechanism to switch complete protein translation from ORF1a to ORF1b, while FUBP3, SLBP, RPL10A, RPS3A, and RPS14 bind directly to the SARS-CoV-2 -1 PRF RNA to promote replication via enhancing the switch procedure. The expression of SFL inhibited SARS-CoV-2 replication via reducing frameshifting. The schematic diagram was generated with www.figdraw.com . Data points represent the mean ± SEM. Multiple comparisons were performed using ANOVA with Dunnett’s test. * P < 0.05, ** P < 0.01, and *** P < 0.001
Oligos Trsa, supplied by Oligos Etc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oligos trsa/product/Oligos Etc
Average 90 stars, based on 1 article reviews
oligos trsa - by Bioz Stars, 2026-02
90/100 stars
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time-resolved near-infrared spectroscopy niro-trs1  (Hamamatsu)
90
Hamamatsu time-resolved near-infrared spectroscopy niro-trs1
SLBP bound to the stem loop region of SARS-CoV-2 -1 PRF RNA. a -1 PRF RNA sequences were conserved among the genome sequences of 19 different SARS-CoV-2 strains. The viral sequences were manually retrieved from the National Center for Biotechnology Information (NCBI). Sequence alignment was conducted using MEGA 11.0 software. b The proposed secondary structure of the SARS-CoV-2 -1 PRF element. c Schematic representations of the -1 PRF RNA studied, including full -1 PRF RNA, ΔSL2 mutant, ΔSL3 mutant, ΔSL1& 2 mutant, and ΔSL2&3 mutant. The schematic diagram was generated with IBS 2.0 ( https://ibs.renlab.org/#/server ). d RNA probes used were obtained from in vitro transcription. e Using cellular proteins extracted from H1299 cells, immunoprecipitation of <t>tRSA-labeled</t> wild-type and mutant -1 PRF RNA probes pulled down SLBP. tRSA-labeled random sequence RNA was used as a negative control ( n = 3). f Interaction between SLBP and wild-type -1 PRF RNA or predicted RNA sequence of -1 PRF RNA using RNA EMSAs. Lane 1, -1 PRF RNA truncated mutant I. Lane 2, -1 PRF RNA truncated mutant II. Lane 3, -1 PRF RNA truncated mutant III. Lane 4, -1 PRF RNA. Lane 5, -1 PRF RNA truncated mutant I + GST. Lane 6, -1 PRF RNA truncated mutant II + GST. Lane 7, -1 PRF RNA truncated mutant III + GST. Lane 8, -1 PRF RNA + GST. Lane 9, -1 PRF RNA truncated mutant I + GST-SLBP. Lane 10, -1 PRF RNA truncated mutant II + GST-SLBP. Lane 11, -1 PRF RNA truncated mutant III + GST-SLBP. Lane 12, -1 PRF RNA + GST-SLBP. Experiments were repeated three times with similar results. g Using cellular proteins extracted from H1299 cells, immunoprecipitation of random sequence RNA, wild-type and silent SL3 mutation -1 PRF RNA probes pulled down SLBP. h Schematic diagram of interaction models between SLBP and -1 PRF RNA through the molecular docking. The RNA pull-down followed by WB assay was performed to evaluate the interaction between SLBP mutants and -1 PRF RNA. Total cell lysates from H1299 cells transfected with different SLBP variants were used in the experiment. These variants included single mutations where arginine 46 was changed to alanine (R46A), serine 94 was changed to alanine (S94A), and a double mutation (R46A & S94A), where both arginine 46 and serine 94 were replaced with alanine, respectively. Meanwhile, total cell lysates from H1299 cells transfected with wild-type SLBP (WT) were used as a positive control. i Co-detection of SLBP with -1 PRF RNA. Polyclonal anti-SLBP was used for SLBP protein immunofluorescence. Scale bar = 20 µm. j Schematic diagram of five host factors promoting SARS-CoV-2 replication via regulating frameshifting. SARS-CoV-2 apply the -1 PRF mechanism to switch complete protein translation from ORF1a to ORF1b, while FUBP3, SLBP, RPL10A, RPS3A, and RPS14 bind directly to the SARS-CoV-2 -1 PRF RNA to promote replication via enhancing the switch procedure. The expression of SFL inhibited SARS-CoV-2 replication via reducing frameshifting. The schematic diagram was generated with www.figdraw.com . Data points represent the mean ± SEM. Multiple comparisons were performed using ANOVA with Dunnett’s test. * P < 0.05, ** P < 0.01, and *** P < 0.001
Time Resolved Near Infrared Spectroscopy Niro Trs1, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/time-resolved near-infrared spectroscopy niro-trs1/product/Hamamatsu
Average 90 stars, based on 1 article reviews
time-resolved near-infrared spectroscopy niro-trs1 - by Bioz Stars, 2026-02
90/100 stars
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gene sequences encoding trcv, trad, trpd, and trsd  (BioCat GmbH)
90
BioCat GmbH gene sequences encoding trcv, trad, trpd, and trsd
SLBP bound to the stem loop region of SARS-CoV-2 -1 PRF RNA. a -1 PRF RNA sequences were conserved among the genome sequences of 19 different SARS-CoV-2 strains. The viral sequences were manually retrieved from the National Center for Biotechnology Information (NCBI). Sequence alignment was conducted using MEGA 11.0 software. b The proposed secondary structure of the SARS-CoV-2 -1 PRF element. c Schematic representations of the -1 PRF RNA studied, including full -1 PRF RNA, ΔSL2 mutant, ΔSL3 mutant, ΔSL1& 2 mutant, and ΔSL2&3 mutant. The schematic diagram was generated with IBS 2.0 ( https://ibs.renlab.org/#/server ). d RNA probes used were obtained from in vitro transcription. e Using cellular proteins extracted from H1299 cells, immunoprecipitation of <t>tRSA-labeled</t> wild-type and mutant -1 PRF RNA probes pulled down SLBP. tRSA-labeled random sequence RNA was used as a negative control ( n = 3). f Interaction between SLBP and wild-type -1 PRF RNA or predicted RNA sequence of -1 PRF RNA using RNA EMSAs. Lane 1, -1 PRF RNA truncated mutant I. Lane 2, -1 PRF RNA truncated mutant II. Lane 3, -1 PRF RNA truncated mutant III. Lane 4, -1 PRF RNA. Lane 5, -1 PRF RNA truncated mutant I + GST. Lane 6, -1 PRF RNA truncated mutant II + GST. Lane 7, -1 PRF RNA truncated mutant III + GST. Lane 8, -1 PRF RNA + GST. Lane 9, -1 PRF RNA truncated mutant I + GST-SLBP. Lane 10, -1 PRF RNA truncated mutant II + GST-SLBP. Lane 11, -1 PRF RNA truncated mutant III + GST-SLBP. Lane 12, -1 PRF RNA + GST-SLBP. Experiments were repeated three times with similar results. g Using cellular proteins extracted from H1299 cells, immunoprecipitation of random sequence RNA, wild-type and silent SL3 mutation -1 PRF RNA probes pulled down SLBP. h Schematic diagram of interaction models between SLBP and -1 PRF RNA through the molecular docking. The RNA pull-down followed by WB assay was performed to evaluate the interaction between SLBP mutants and -1 PRF RNA. Total cell lysates from H1299 cells transfected with different SLBP variants were used in the experiment. These variants included single mutations where arginine 46 was changed to alanine (R46A), serine 94 was changed to alanine (S94A), and a double mutation (R46A & S94A), where both arginine 46 and serine 94 were replaced with alanine, respectively. Meanwhile, total cell lysates from H1299 cells transfected with wild-type SLBP (WT) were used as a positive control. i Co-detection of SLBP with -1 PRF RNA. Polyclonal anti-SLBP was used for SLBP protein immunofluorescence. Scale bar = 20 µm. j Schematic diagram of five host factors promoting SARS-CoV-2 replication via regulating frameshifting. SARS-CoV-2 apply the -1 PRF mechanism to switch complete protein translation from ORF1a to ORF1b, while FUBP3, SLBP, RPL10A, RPS3A, and RPS14 bind directly to the SARS-CoV-2 -1 PRF RNA to promote replication via enhancing the switch procedure. The expression of SFL inhibited SARS-CoV-2 replication via reducing frameshifting. The schematic diagram was generated with www.figdraw.com . Data points represent the mean ± SEM. Multiple comparisons were performed using ANOVA with Dunnett’s test. * P < 0.05, ** P < 0.01, and *** P < 0.001
Gene Sequences Encoding Trcv, Trad, Trpd, And Trsd, supplied by BioCat GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
gene sequences encoding trcv, trad, trpd, and trsd - by Bioz Stars, 2026-02
90/100 stars
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trsc-labeled donkey anti–mouse igg f(ab′) 2  (Jackson Immuno)
90
Jackson Immuno trsc-labeled donkey anti–mouse igg f(ab′) 2
SLBP bound to the stem loop region of SARS-CoV-2 -1 PRF RNA. a -1 PRF RNA sequences were conserved among the genome sequences of 19 different SARS-CoV-2 strains. The viral sequences were manually retrieved from the National Center for Biotechnology Information (NCBI). Sequence alignment was conducted using MEGA 11.0 software. b The proposed secondary structure of the SARS-CoV-2 -1 PRF element. c Schematic representations of the -1 PRF RNA studied, including full -1 PRF RNA, ΔSL2 mutant, ΔSL3 mutant, ΔSL1& 2 mutant, and ΔSL2&3 mutant. The schematic diagram was generated with IBS 2.0 ( https://ibs.renlab.org/#/server ). d RNA probes used were obtained from in vitro transcription. e Using cellular proteins extracted from H1299 cells, immunoprecipitation of <t>tRSA-labeled</t> wild-type and mutant -1 PRF RNA probes pulled down SLBP. tRSA-labeled random sequence RNA was used as a negative control ( n = 3). f Interaction between SLBP and wild-type -1 PRF RNA or predicted RNA sequence of -1 PRF RNA using RNA EMSAs. Lane 1, -1 PRF RNA truncated mutant I. Lane 2, -1 PRF RNA truncated mutant II. Lane 3, -1 PRF RNA truncated mutant III. Lane 4, -1 PRF RNA. Lane 5, -1 PRF RNA truncated mutant I + GST. Lane 6, -1 PRF RNA truncated mutant II + GST. Lane 7, -1 PRF RNA truncated mutant III + GST. Lane 8, -1 PRF RNA + GST. Lane 9, -1 PRF RNA truncated mutant I + GST-SLBP. Lane 10, -1 PRF RNA truncated mutant II + GST-SLBP. Lane 11, -1 PRF RNA truncated mutant III + GST-SLBP. Lane 12, -1 PRF RNA + GST-SLBP. Experiments were repeated three times with similar results. g Using cellular proteins extracted from H1299 cells, immunoprecipitation of random sequence RNA, wild-type and silent SL3 mutation -1 PRF RNA probes pulled down SLBP. h Schematic diagram of interaction models between SLBP and -1 PRF RNA through the molecular docking. The RNA pull-down followed by WB assay was performed to evaluate the interaction between SLBP mutants and -1 PRF RNA. Total cell lysates from H1299 cells transfected with different SLBP variants were used in the experiment. These variants included single mutations where arginine 46 was changed to alanine (R46A), serine 94 was changed to alanine (S94A), and a double mutation (R46A & S94A), where both arginine 46 and serine 94 were replaced with alanine, respectively. Meanwhile, total cell lysates from H1299 cells transfected with wild-type SLBP (WT) were used as a positive control. i Co-detection of SLBP with -1 PRF RNA. Polyclonal anti-SLBP was used for SLBP protein immunofluorescence. Scale bar = 20 µm. j Schematic diagram of five host factors promoting SARS-CoV-2 replication via regulating frameshifting. SARS-CoV-2 apply the -1 PRF mechanism to switch complete protein translation from ORF1a to ORF1b, while FUBP3, SLBP, RPL10A, RPS3A, and RPS14 bind directly to the SARS-CoV-2 -1 PRF RNA to promote replication via enhancing the switch procedure. The expression of SFL inhibited SARS-CoV-2 replication via reducing frameshifting. The schematic diagram was generated with www.figdraw.com . Data points represent the mean ± SEM. Multiple comparisons were performed using ANOVA with Dunnett’s test. * P < 0.05, ** P < 0.01, and *** P < 0.001
Trsc Labeled Donkey Anti–Mouse Igg F(Ab′) 2, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
trsc-labeled donkey anti–mouse igg f(ab′) 2 - by Bioz Stars, 2026-02
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transaminases trad, trcv, trpd, and trsd  (BioCat GmbH)
90
BioCat GmbH transaminases trad, trcv, trpd, and trsd
SLBP bound to the stem loop region of SARS-CoV-2 -1 PRF RNA. a -1 PRF RNA sequences were conserved among the genome sequences of 19 different SARS-CoV-2 strains. The viral sequences were manually retrieved from the National Center for Biotechnology Information (NCBI). Sequence alignment was conducted using MEGA 11.0 software. b The proposed secondary structure of the SARS-CoV-2 -1 PRF element. c Schematic representations of the -1 PRF RNA studied, including full -1 PRF RNA, ΔSL2 mutant, ΔSL3 mutant, ΔSL1& 2 mutant, and ΔSL2&3 mutant. The schematic diagram was generated with IBS 2.0 ( https://ibs.renlab.org/#/server ). d RNA probes used were obtained from in vitro transcription. e Using cellular proteins extracted from H1299 cells, immunoprecipitation of <t>tRSA-labeled</t> wild-type and mutant -1 PRF RNA probes pulled down SLBP. tRSA-labeled random sequence RNA was used as a negative control ( n = 3). f Interaction between SLBP and wild-type -1 PRF RNA or predicted RNA sequence of -1 PRF RNA using RNA EMSAs. Lane 1, -1 PRF RNA truncated mutant I. Lane 2, -1 PRF RNA truncated mutant II. Lane 3, -1 PRF RNA truncated mutant III. Lane 4, -1 PRF RNA. Lane 5, -1 PRF RNA truncated mutant I + GST. Lane 6, -1 PRF RNA truncated mutant II + GST. Lane 7, -1 PRF RNA truncated mutant III + GST. Lane 8, -1 PRF RNA + GST. Lane 9, -1 PRF RNA truncated mutant I + GST-SLBP. Lane 10, -1 PRF RNA truncated mutant II + GST-SLBP. Lane 11, -1 PRF RNA truncated mutant III + GST-SLBP. Lane 12, -1 PRF RNA + GST-SLBP. Experiments were repeated three times with similar results. g Using cellular proteins extracted from H1299 cells, immunoprecipitation of random sequence RNA, wild-type and silent SL3 mutation -1 PRF RNA probes pulled down SLBP. h Schematic diagram of interaction models between SLBP and -1 PRF RNA through the molecular docking. The RNA pull-down followed by WB assay was performed to evaluate the interaction between SLBP mutants and -1 PRF RNA. Total cell lysates from H1299 cells transfected with different SLBP variants were used in the experiment. These variants included single mutations where arginine 46 was changed to alanine (R46A), serine 94 was changed to alanine (S94A), and a double mutation (R46A & S94A), where both arginine 46 and serine 94 were replaced with alanine, respectively. Meanwhile, total cell lysates from H1299 cells transfected with wild-type SLBP (WT) were used as a positive control. i Co-detection of SLBP with -1 PRF RNA. Polyclonal anti-SLBP was used for SLBP protein immunofluorescence. Scale bar = 20 µm. j Schematic diagram of five host factors promoting SARS-CoV-2 replication via regulating frameshifting. SARS-CoV-2 apply the -1 PRF mechanism to switch complete protein translation from ORF1a to ORF1b, while FUBP3, SLBP, RPL10A, RPS3A, and RPS14 bind directly to the SARS-CoV-2 -1 PRF RNA to promote replication via enhancing the switch procedure. The expression of SFL inhibited SARS-CoV-2 replication via reducing frameshifting. The schematic diagram was generated with www.figdraw.com . Data points represent the mean ± SEM. Multiple comparisons were performed using ANOVA with Dunnett’s test. * P < 0.05, ** P < 0.01, and *** P < 0.001
Transaminases Trad, Trcv, Trpd, And Trsd, supplied by BioCat GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transaminases trad, trcv, trpd, and trsd/product/BioCat GmbH
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transaminases trad, trcv, trpd, and trsd - by Bioz Stars, 2026-02
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trsv  (Kamada)
90
Kamada trsv
SLBP bound to the stem loop region of SARS-CoV-2 -1 PRF RNA. a -1 PRF RNA sequences were conserved among the genome sequences of 19 different SARS-CoV-2 strains. The viral sequences were manually retrieved from the National Center for Biotechnology Information (NCBI). Sequence alignment was conducted using MEGA 11.0 software. b The proposed secondary structure of the SARS-CoV-2 -1 PRF element. c Schematic representations of the -1 PRF RNA studied, including full -1 PRF RNA, ΔSL2 mutant, ΔSL3 mutant, ΔSL1& 2 mutant, and ΔSL2&3 mutant. The schematic diagram was generated with IBS 2.0 ( https://ibs.renlab.org/#/server ). d RNA probes used were obtained from in vitro transcription. e Using cellular proteins extracted from H1299 cells, immunoprecipitation of <t>tRSA-labeled</t> wild-type and mutant -1 PRF RNA probes pulled down SLBP. tRSA-labeled random sequence RNA was used as a negative control ( n = 3). f Interaction between SLBP and wild-type -1 PRF RNA or predicted RNA sequence of -1 PRF RNA using RNA EMSAs. Lane 1, -1 PRF RNA truncated mutant I. Lane 2, -1 PRF RNA truncated mutant II. Lane 3, -1 PRF RNA truncated mutant III. Lane 4, -1 PRF RNA. Lane 5, -1 PRF RNA truncated mutant I + GST. Lane 6, -1 PRF RNA truncated mutant II + GST. Lane 7, -1 PRF RNA truncated mutant III + GST. Lane 8, -1 PRF RNA + GST. Lane 9, -1 PRF RNA truncated mutant I + GST-SLBP. Lane 10, -1 PRF RNA truncated mutant II + GST-SLBP. Lane 11, -1 PRF RNA truncated mutant III + GST-SLBP. Lane 12, -1 PRF RNA + GST-SLBP. Experiments were repeated three times with similar results. g Using cellular proteins extracted from H1299 cells, immunoprecipitation of random sequence RNA, wild-type and silent SL3 mutation -1 PRF RNA probes pulled down SLBP. h Schematic diagram of interaction models between SLBP and -1 PRF RNA through the molecular docking. The RNA pull-down followed by WB assay was performed to evaluate the interaction between SLBP mutants and -1 PRF RNA. Total cell lysates from H1299 cells transfected with different SLBP variants were used in the experiment. These variants included single mutations where arginine 46 was changed to alanine (R46A), serine 94 was changed to alanine (S94A), and a double mutation (R46A & S94A), where both arginine 46 and serine 94 were replaced with alanine, respectively. Meanwhile, total cell lysates from H1299 cells transfected with wild-type SLBP (WT) were used as a positive control. i Co-detection of SLBP with -1 PRF RNA. Polyclonal anti-SLBP was used for SLBP protein immunofluorescence. Scale bar = 20 µm. j Schematic diagram of five host factors promoting SARS-CoV-2 replication via regulating frameshifting. SARS-CoV-2 apply the -1 PRF mechanism to switch complete protein translation from ORF1a to ORF1b, while FUBP3, SLBP, RPL10A, RPS3A, and RPS14 bind directly to the SARS-CoV-2 -1 PRF RNA to promote replication via enhancing the switch procedure. The expression of SFL inhibited SARS-CoV-2 replication via reducing frameshifting. The schematic diagram was generated with www.figdraw.com . Data points represent the mean ± SEM. Multiple comparisons were performed using ANOVA with Dunnett’s test. * P < 0.05, ** P < 0.01, and *** P < 0.001
Trsv, supplied by Kamada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trsv/product/Kamada
Average 90 stars, based on 1 article reviews
trsv - by Bioz Stars, 2026-02
90/100 stars
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SLBP bound to the stem loop region of SARS-CoV-2 -1 PRF RNA. a -1 PRF RNA sequences were conserved among the genome sequences of 19 different SARS-CoV-2 strains. The viral sequences were manually retrieved from the National Center for Biotechnology Information (NCBI). Sequence alignment was conducted using MEGA 11.0 software. b The proposed secondary structure of the SARS-CoV-2 -1 PRF element. c Schematic representations of the -1 PRF RNA studied, including full -1 PRF RNA, ΔSL2 mutant, ΔSL3 mutant, ΔSL1& 2 mutant, and ΔSL2&3 mutant. The schematic diagram was generated with IBS 2.0 ( https://ibs.renlab.org/#/server ). d RNA probes used were obtained from in vitro transcription. e Using cellular proteins extracted from H1299 cells, immunoprecipitation of tRSA-labeled wild-type and mutant -1 PRF RNA probes pulled down SLBP. tRSA-labeled random sequence RNA was used as a negative control ( n = 3). f Interaction between SLBP and wild-type -1 PRF RNA or predicted RNA sequence of -1 PRF RNA using RNA EMSAs. Lane 1, -1 PRF RNA truncated mutant I. Lane 2, -1 PRF RNA truncated mutant II. Lane 3, -1 PRF RNA truncated mutant III. Lane 4, -1 PRF RNA. Lane 5, -1 PRF RNA truncated mutant I + GST. Lane 6, -1 PRF RNA truncated mutant II + GST. Lane 7, -1 PRF RNA truncated mutant III + GST. Lane 8, -1 PRF RNA + GST. Lane 9, -1 PRF RNA truncated mutant I + GST-SLBP. Lane 10, -1 PRF RNA truncated mutant II + GST-SLBP. Lane 11, -1 PRF RNA truncated mutant III + GST-SLBP. Lane 12, -1 PRF RNA + GST-SLBP. Experiments were repeated three times with similar results. g Using cellular proteins extracted from H1299 cells, immunoprecipitation of random sequence RNA, wild-type and silent SL3 mutation -1 PRF RNA probes pulled down SLBP. h Schematic diagram of interaction models between SLBP and -1 PRF RNA through the molecular docking. The RNA pull-down followed by WB assay was performed to evaluate the interaction between SLBP mutants and -1 PRF RNA. Total cell lysates from H1299 cells transfected with different SLBP variants were used in the experiment. These variants included single mutations where arginine 46 was changed to alanine (R46A), serine 94 was changed to alanine (S94A), and a double mutation (R46A & S94A), where both arginine 46 and serine 94 were replaced with alanine, respectively. Meanwhile, total cell lysates from H1299 cells transfected with wild-type SLBP (WT) were used as a positive control. i Co-detection of SLBP with -1 PRF RNA. Polyclonal anti-SLBP was used for SLBP protein immunofluorescence. Scale bar = 20 µm. j Schematic diagram of five host factors promoting SARS-CoV-2 replication via regulating frameshifting. SARS-CoV-2 apply the -1 PRF mechanism to switch complete protein translation from ORF1a to ORF1b, while FUBP3, SLBP, RPL10A, RPS3A, and RPS14 bind directly to the SARS-CoV-2 -1 PRF RNA to promote replication via enhancing the switch procedure. The expression of SFL inhibited SARS-CoV-2 replication via reducing frameshifting. The schematic diagram was generated with www.figdraw.com . Data points represent the mean ± SEM. Multiple comparisons were performed using ANOVA with Dunnett’s test. * P < 0.05, ** P < 0.01, and *** P < 0.001

Journal: Signal Transduction and Targeted Therapy

Article Title: Stem loop binding protein promotes SARS-CoV-2 replication via -1 programmed ribosomal frameshifting

doi: 10.1038/s41392-025-02277-w

Figure Lengend Snippet: SLBP bound to the stem loop region of SARS-CoV-2 -1 PRF RNA. a -1 PRF RNA sequences were conserved among the genome sequences of 19 different SARS-CoV-2 strains. The viral sequences were manually retrieved from the National Center for Biotechnology Information (NCBI). Sequence alignment was conducted using MEGA 11.0 software. b The proposed secondary structure of the SARS-CoV-2 -1 PRF element. c Schematic representations of the -1 PRF RNA studied, including full -1 PRF RNA, ΔSL2 mutant, ΔSL3 mutant, ΔSL1& 2 mutant, and ΔSL2&3 mutant. The schematic diagram was generated with IBS 2.0 ( https://ibs.renlab.org/#/server ). d RNA probes used were obtained from in vitro transcription. e Using cellular proteins extracted from H1299 cells, immunoprecipitation of tRSA-labeled wild-type and mutant -1 PRF RNA probes pulled down SLBP. tRSA-labeled random sequence RNA was used as a negative control ( n = 3). f Interaction between SLBP and wild-type -1 PRF RNA or predicted RNA sequence of -1 PRF RNA using RNA EMSAs. Lane 1, -1 PRF RNA truncated mutant I. Lane 2, -1 PRF RNA truncated mutant II. Lane 3, -1 PRF RNA truncated mutant III. Lane 4, -1 PRF RNA. Lane 5, -1 PRF RNA truncated mutant I + GST. Lane 6, -1 PRF RNA truncated mutant II + GST. Lane 7, -1 PRF RNA truncated mutant III + GST. Lane 8, -1 PRF RNA + GST. Lane 9, -1 PRF RNA truncated mutant I + GST-SLBP. Lane 10, -1 PRF RNA truncated mutant II + GST-SLBP. Lane 11, -1 PRF RNA truncated mutant III + GST-SLBP. Lane 12, -1 PRF RNA + GST-SLBP. Experiments were repeated three times with similar results. g Using cellular proteins extracted from H1299 cells, immunoprecipitation of random sequence RNA, wild-type and silent SL3 mutation -1 PRF RNA probes pulled down SLBP. h Schematic diagram of interaction models between SLBP and -1 PRF RNA through the molecular docking. The RNA pull-down followed by WB assay was performed to evaluate the interaction between SLBP mutants and -1 PRF RNA. Total cell lysates from H1299 cells transfected with different SLBP variants were used in the experiment. These variants included single mutations where arginine 46 was changed to alanine (R46A), serine 94 was changed to alanine (S94A), and a double mutation (R46A & S94A), where both arginine 46 and serine 94 were replaced with alanine, respectively. Meanwhile, total cell lysates from H1299 cells transfected with wild-type SLBP (WT) were used as a positive control. i Co-detection of SLBP with -1 PRF RNA. Polyclonal anti-SLBP was used for SLBP protein immunofluorescence. Scale bar = 20 µm. j Schematic diagram of five host factors promoting SARS-CoV-2 replication via regulating frameshifting. SARS-CoV-2 apply the -1 PRF mechanism to switch complete protein translation from ORF1a to ORF1b, while FUBP3, SLBP, RPL10A, RPS3A, and RPS14 bind directly to the SARS-CoV-2 -1 PRF RNA to promote replication via enhancing the switch procedure. The expression of SFL inhibited SARS-CoV-2 replication via reducing frameshifting. The schematic diagram was generated with www.figdraw.com . Data points represent the mean ± SEM. Multiple comparisons were performed using ANOVA with Dunnett’s test. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: To generate RNA pull-down probes, pcDNA3.0-random sequence-tRSA, pcDNA3.0-(-1 FSE)-tRSA, pcDNA3.0-(-1 PRF)-tRSA, pcDNA3.0-(-1 PRF Δ2)-tRSA, pcDNA3.0-(-1 PRF Δ3)-tRSA, pcDNA3.0-(-1 PRF Δ1& Δ2)-tRSA, pcDNA3.0-(-1 PRF Δ2& Δ3)-tRSA, and pcDNA3.0-(-1 PRF Mutation)-tRSA were linearized by EcoRV (#R3195L, NEB) digestion, followed by in vitro transcription utilizing T7 RiboMAXTM Large Scale RNA Production Systems (P1300, Promega).

Techniques: Sequencing, Software, Mutagenesis, Generated, In Vitro, Immunoprecipitation, Labeling, Negative Control, Transfection, Positive Control, Immunofluorescence, Expressing